moloX focusses on the production of highly pure proteins. We offer custom protein production in bacterial, insect and mammalian cell expression systems and subsequent protein purification up to crystallization grade. We offer uniform isotope labeling such as 15N or 13C for NMR purposes.
moloX holds an outstanding expertise in protein expression and purification of challenging multi-subunit protein and protein-nucleic acid complexes. On customer demand the protein production could be scaled up from µg to mg range.
Additionally we can perform isothermal titration calorimetry (ITC) to measure binding affinities of protein-protein interactions or binding of small molecules to protein targets.
Furthermore we can offer buffer optimization to increase protein stability.
On customer demand we can perform crystallization experiments in the nanoliter range with a state of the art high-throughput crystallization platform. If necessary we can perform construct optimization to increase the crystallizability of target proteins. Upon request moloX determines 3D-structures of target proteins by X-ray crystallography.